![]() ![]() One of the authors (NP) was previously employed by TriLink BioTechnologies, LLC but is currently at Synthetic Genomics, Inc. did not have any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: Several of the authors (SS, JMH, AL, MPS, GZ, APM, RIH) are employed by a commercial company: TriLink BioTechnologies, LLC where GZ is a consultant and RIH is the CEO. TriLink BioTechnologies, LLC provided support in the form of salaries for authors after grant funding had ended (SS, JMH, AL, MS, GZ, APM, NP, RIH) and did have a role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Several of the authors (SS, JMH, AL, MS, GZ, APM, RIH) are employed by a commercial company: TriLink BioTechnologies, LLC. The specific roles of these authors are articulated in the ‘author contributions’ section. The funder provided support in the form of salaries for authors (SS, AL, MS, GZ, NP, RIH), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files or FASTQ files are available from the NCBI Short Reads Archive database as Bioproject (PRJNA350292) with accession numbers (SAMN05942029 - SAMN05942088).įunding: This research was funded with a Small Business Innovation Research (SBIR) grant through the National Institutes of Health (NIH), Grant Number: 1R43HG006820-01A1. Received: AugAccepted: NovemPublished: November 22, 2016Ĭopyright: © 2016 Shore et al. PLoS ONE 11(11):Įditor: Ramamurthy Mahalingam, USDA Agricultural Research Service, UNITED STATES (2016) Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation. ![]() This workflow allows for lower sample inputs and elimination of the gel purification step, which in turn allows for an automatable sRNA library preparation protocol.Ĭitation: Shore S, Henderson JM, Lebedev A, Salcedo MP, Zon G, McCaffrey AP, et al. Here we address the need for improved specificity of sRNA library preparation workflows with a novel library preparation approach that uses modified adapters to suppress adapter dimer formation. At very low sample inputs, adapter dimer side products dominate the reaction and limit the sensitivity of this technique. Most sRNA library preparation strategies thus employ a gel purification step to isolate tagged library from adapter dimer contaminants. These side products, known as adapter dimer, are very similar in size to the tagged library. Greater adoption of small RNA (sRNA) sequencing has been hindered by high sample input requirements and inherent ligation side products formed during library preparation. For most sample types, the automation of RNA and DNA sample preparation workflows enables high throughput next-generation sequencing (NGS) library preparation. ![]()
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